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Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (ab250412)

Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (ab250412)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13391] to SPAG7 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-SPAG7 antibody [EPR13391] - BSA and Azide free
    See all SPAG7 primary antibodies
  • Description

    Rabbit monoclonal [EPR13391] to SPAG7 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250412 is the carrier-free version of ab181187. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250412 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13391
  • Isotype

    IgG

Images

  • Western blot - Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (ab250412)
    Western blot - Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (ab250412)
    All lanes : Anti-SPAG7 antibody [EPR13391] (ab181187) at 1/5000 dilution

    Lane 1 : MCF-7 cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/5000 dilution

    Predicted band size: 26 kDa



    This data was developed using ab181187, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (ab250412)
    Immunoprecipitation - Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (ab250412)

    This data was developed using ab181187, the same antibody clone in a different buffer formulation.

    Immunoprecipitation. ab181187 at 1/1000 labeling SPAG7 immunoprecipitated from HeLa cell lysate using ab181187 at 1/50.

    Lane 2: Control IgG.

  • Immunocytochemistry - Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (ab250412)
    Immunocytochemistry - Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (ab250412)
    This data was developed using ab181187, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of MCF7 cells labeling SPAG7 with ab181187 at 1/250 (green) with Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 and DAPI staining (blue).
  • Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (ab250412)
    Anti-SPAG7 antibody [EPR13391] - BSA and Azide free (ab250412)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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