Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UMB4] to Somatostatin Receptor 5
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Somatostatin Receptor 5 antibody [UMB4]
    See all Somatostatin Receptor 5 primary antibodies

  • Description

    Rabbit monoclonal [UMB4] to Somatostatin Receptor 5

  • Host species

    Rabbit

  • Specificity

    Internal WB test in PANC-1, human pancreas and human brain lysates indicates this antibody might not detect endogenous Somatostatin Receptor 5.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab178801)

  • Positive control

    • WB: HT29 whole cell lysate (ab3952), 293T (Human embryonic kidney epithelial cell) transfected with human SSTR5 expression vector, IHC-P: Human pancreas

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreas tissue sections labeling Somatostatin Receptor 5 with Purified ab109495 at 1:50 dilution (16.3 µg/ml). Heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.. Performed on a Leica Biosystems BOND ® RX instrument.was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495) Kaemmerer, D. et al BMC Cancer. 2017 Dec 28;17(1):896. doi: 10.1186/s12885-017-3911-3 Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

    Somatostatin receptor 2 (SSTR2), somatostatin receptor 5 (SSTR5) and CXCR4 expression in the hepatoblastoma cell line HepG2 and in the hepatoma cell lines Hep3B and HuH-7 in comparison to the small cell lung cancer cell line NCI-H69 and the neuroendocrine tumor cell line BON-1. Immunohistochemistry (red-brown color), counterstaining with hematoxylin; scale bar: 20 μm. Representative photomicrographs of three independent batches are shown.

    HepG2, Hep3B, HuH-7, NCI-H-69, and BON-1 cells (DSMZ, Braunschweig, Germany) were grown in 75 cm2 culture flasks to a confluency of 80%. Cells were washed once with phosphate-buffered saline and transferred into 10% buffered formalin (J.T.Baker, Deventer, The Netherlands) for 2 h. After centrifugation for 10 min at 3500 x g, the supernatant was removed, and 1 ml human pool plasma was added to the cell samples. After brief vortexing, 100 μl human fibrinogen (50–70% protein; ≥80% clottable) was added to each sample, and the samples were vortexed again. The resulting clots were placed for another 24 h in 10% buffered formalin and embedded in paraffin blocks.

    (After Kaemmerer, D. et al BMC Cancer. 2017 Dec 28;17(1):896. doi: 10.1186/s12885-017-3911-3).

    Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/)

     

  • Western blot - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
    Western blot - Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
    Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495) at 0.08 µg/ml (purified) + 293T (Human embryonic kidney epithelial cell) transfected with human SSTR5 expression vector, whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 39 kDa



    Blocking and diluting buffer: 5% NFDM/TBST
  • Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)
    Anti-Somatostatin Receptor 5 antibody [UMB4] (ab109495)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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