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Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)

Key features and details

  • Mouse monoclonal [OTI3D3] to SOCS3 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Human, African green monkey
  • Isotype: IgG2a

Overview

  • Product name

    Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free
    See all SOCS3 primary antibodies

  • Description

    Mouse monoclonal [OTI3D3] to SOCS3 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human, African green monkey

  • Immunogen

    Recombinant full length protein corresponding to Human SOCS3 aa 1-250.
    Database link: O14543

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Transfected HEK-293T cell lysate; IHC-P: human colon adenocarcinoma, thyroid carcinoma tissue; IF: Transfected COS7 whole cells; Flow: HeLa and Jurkat whole cells.

  • General notes

    ab273652 is the carrier-free version of ab236519. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)
    Western blot - Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)
    All lanes : Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : pCMV6-ENTRY SOCS3-transfected HEK-293T whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 24 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)

    Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma tissue staining SOCS3 with ab236519 at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236519).

  • Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)
    Immunocytochemistry/ Immunofluorescence - Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)

    Immunofluorescence analysis of COS-7 (african green monkey kidney fibroblast-like cell line) whole cells labeling SOCS3 with ab236519 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236519).

  • Flow Cytometry - Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)
    Flow Cytometry - Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)

    Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell using ab236519 (Red) at 1/100 dilution, compared to a nonspecific negative control antibody, (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236519).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue staining SOCS3 with ab236519 at 1/150 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236519).

  • Flow Cytometry - Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)
    Flow Cytometry - Anti-SOCS3 antibody [OTI3D3] - BSA and Azide free (ab273652)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell using ab236519 (Red) at 1/100 dilution, compared to a nonspecific negative control antibody, (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236519).

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