Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11106(2)] to SMYD3 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC, IP, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free
    See all SMYD3 primary antibodies

  • Description

    Rabbit monoclonal [EPR11106(2)] to SMYD3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, ICC, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HAP1, 293, MCF-7 and T47-D cell lysates. IHC-P: Human testis and skeletal muscle tissue. ICC: HeLa cells. Flow Cyt (intra): 293 cells.

  • General notes

    ab250657 is the carrier-free version of ab183498.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    Western blot - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    All lanes : Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : SMYD3 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 49 kDa



    This data was developed using ab183498, the same antibody clone in a different buffer formulation.

    Lanes 1 - 4: Merged signal (red and green). Green - ab183498 observed at 49 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab183498 was shown to recognize SMYD3 in wild-type HAP1 cells as signal was lost at the expected MW in SMYD3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SMYD3 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab183498 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    Western blot - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    All lanes : Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : 293 lysate
    Lane 3 : T47-D lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 49 kDa



    This data was developed using ab183498, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)

    This data was developed using ab183498, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling SMYD3 at 1/100 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    Immunocytochemistry - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    This data was developed using ab183498, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells fixed in 20 °C acetone labeling SMYD3 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 (red). Counterstained with DAPI (blue)
  • Western blot - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    Western blot - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    All lanes : Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : SMYD3 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 49 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183498).

    Lanes 1 - 4: Merged signal (red and green). Green - ab183498 observed at 49 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab183498 was shown to recognize SMYD3 in wild-type HAP1 cells as signal was lost at the expected MW in SMYD3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SMYD3 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab183498 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    Immunoprecipitation - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    This data was developed using ab183498, the same antibody clone in a different buffer formulation.Immunoprecipitation of HeLa cells labeling SMYD3 with ab183498 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.
  • Flow Cytometry - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    Flow Cytometry - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    This data was developed using ab183498, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 293 cells fixed in 2% paraformaldehyde labeling SMYD3 with ab183498 at 1/110 dilution and Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit Monoclonal IgG was used as an isotype control.
  • Western blot - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    Western blot - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/2000 dilution + MCF-7 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 49 kDa



    This data was developed using ab183498, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    This data was developed using ab183498, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis labeling SMYD3 with ab183498 at 1/100 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)
    Anti-SMYD3 antibody [EPR11106(2)] - BSA and Azide free (ab250657)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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