Anti-SMPDL3B antibody (ab30912)
Key features and details
- Rabbit polyclonal to SMPDL3B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMPDL3B antibody -
Description
Rabbit polyclonal to SMPDL3B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human SMPDL3B aa 50-150 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Numbering is based off mature sequence
Database link: Q92485 -
Positive control
- WB: Human fetal lung lysate; HT1080 cell lysate. IHC-P: Human intestine tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was purified by peptide affinity chromatography method. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SMPDL3B antibody (ab30912)Anti-SMPDL3B antibody (ab30912) at 1 µg/ml + Human Fetal Lung at 10 µg
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Western blot analysis of human fetal lung lysate labeling SMPDL3B with ab30912.
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Western blot - Anti-SMPDL3B antibody (ab30912)Anti-SMPDL3B antibody (ab30912) at 1 µg/ml + HT1080 Cell Lysate at 10 µg
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Western blot analysis of HT1080 cell lysate labeling SMPDL3B with ab30912.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMPDL3B antibody (ab30912)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling SMPDL3B with ab30912 at 4-8µg/ml. Arrows indicate positive staining of epithelial cells of intestinal villus. Magnification: 400X.