Anti-SMEK2 antibody (ab224222)
Key features and details
- Rabbit polyclonal to SMEK2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SMEK2 antibody
See all SMEK2 primary antibodies -
Description
Rabbit polyclonal to SMEK2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human SMEK2 aa 650-750. aa729-732 are modified.
Database link: Q5MIZ7 -
Positive control
- WB: NIH/3T3, NBT-II, RT4, EFO-21 and A431 cell lysates. ICC/IF: U-251 MG cells. IHC-P: Human cerebral cortex, testis and fallopian tube tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (ab224222)
Paraffin-embedded human fallopian tube tissue stained for SMEK2 using ab224222 at 1/50 dilution in immunohistochemical analysis. Shows strong nuclear positivity in glandular cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (ab224222)
Paraffin-embedded human testis tissue stained for SMEK2 using ab224222 at 1/50 dilution in immunohistochemical analysis. Shows strong nuclear postitivity in seminiferous ducts cells.
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PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for SMEK2 (green) using ab224222 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (ab224222)
Paraffin-embedded human cerebral cortex tissue stained for SMEK2 using ab224222 at 1/50 dilution in immunohistochemical analysis.
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All lanes : Anti-SMEK2 antibody (ab224222) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : EFO-21 cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 97 kDa
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All lanes : Anti-SMEK2 antibody (ab224222) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 97 kDa