Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [2C5] to Serum Response Factor SRF
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Serum Response Factor SRF antibody [2C5]
    See all Serum Response Factor SRF primary antibodies

  • Description

    Rat monoclonal [2C5] to Serum Response Factor SRF

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    IP
    Mouse
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, NIH/3T3, PC-12, HEK-293, K562 and C2C12 whole cell lysates. IP: HeLa and C2C12 whole cell lysates. IHC-P: Mouse cerebrum tissue. Mouse and rat skeletal muscle tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
    Western blot - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
    All lanes : Anti-Serum Response Factor SRF antibody [2C5] (ab252868) at 0.082 µg/ml

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 52 kDa
    Observed band size: 57,67 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 26 seconds; Lane 2: 3 minutes.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590 and PMID: 10642500).

  • Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
    Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

    Serum Response Factor SRF was immunoprecipitated from C2C12 (mouse myoblasts myoblast), whole cell lysate with ab252868 at 1/30 dilution (2µg in 0.35mg lysates) (36.067µg/ml). Western blot was performed on the immunoprecipitate using ab252868 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (ab205720) was used at 1/10000 dilution.

    Lane 1: C2C12 whole cell lysate 10µg.

    Lane 2: ab252868 IP in C2C12 whole cell lysate.

    Lane 3: Rat monoclonal IgG2a (ab18450) instead of ab252868 in C2C12 whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 6 seconds.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590 and PMID: 10642500).

  • Western blot - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
    Western blot - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
    All lanes : Anti-Serum Response Factor SRF antibody [2C5] (ab252868) at 1.082 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
    Lane 4 : C2C12 (mouse myoblasts myoblast), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52,57,67 kDa why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590 and PMID: 10642500). 

  • Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
    Immunoprecipitation - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

    Serum Response Factor SRF was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab252868 at 1/30 dilution (2µg in 0.35mg lysates) (36.067µg/ml). Western blot was performed on the immunoprecipitate using ab252868 at 1/1000 dilution. Goat Anti-rat IgG (H+L), HRP) (ab205720) was used at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10µg.

    Lane 2: ab252868 IP in HeLa whole cell lysate.

    Lane 3: Rat monoclonal IgG2a (ab18450) instead of ab252868 in HeLa whole cell lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11893590and PMID: 10642500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Serum Response Factor SRF with ab252868 at 1/1000 dilution (1.082µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse cerebrum. The section was incubated with ab252868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Serum Response Factor SRF with ab252868 at 1/1000 dilution (1.082µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse skeletal muscle tissue. The section was incubated with ab252868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Serum Response Factor SRF with ab252868 at 1/1000 dilution (1.082µg/ml) followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat skeletal muscle tissue. The section was incubated with ab252868 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Serum Response Factor SRF antibody [2C5] (ab252868)
    Anti-Serum Response Factor SRF antibody [2C5] (ab252868)

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