Anti-SEL1L antibody (ab78298)
Key features and details
- Rabbit polyclonal to SEL1L
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SEL1L antibody -
Description
Rabbit polyclonal to SEL1L -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human SEL1L aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab86659)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatHamsterApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 89 kDa).SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753).
Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 89 kDa).SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753).
Target
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Function
May play a role in Notch signaling (By similarity). May be involved in the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. -
Tissue specificity
Highly expressed in pancreas. -
Sequence similarities
Belongs to the sel-1 family.
Contains 1 fibronectin type-II domain.
Contains 11 Sel1-like repeats. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 6400 Human
- Entrez Gene: 20338 Mouse
- Entrez Gene: 116488 Rat
- Entrez Gene: 314352 Rat
- Omim: 602329 Human
- SwissProt: Q12916 Human
- SwissProt: Q9UBV2 Human
- SwissProt: Q9Z2G6 Mouse
see all -
Alternative names
- IBD2 antibody
- PRO1063 antibody
- Protein sel-1 homolog 1 antibody
see all
Images
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Western blot - Anti-SEL1L antibody (ab78298)Anti-SEL1L antibody (ab78298) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab78298 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753).
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Western blot - Anti-SEL1L antibody (ab78298)This image is courtesy of an abreview submitted by Antibody Solutions Ltd.All lanes : Anti-SEL1L antibody (ab78298) at 1 µg/ml
Lane 1 : Mouse Pancreas
Lane 2 : Mouse Liver
Lane 3 : Mouse Heart
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Swine anti-Rabbit HRP at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 24& 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Datasheets and documents
References (14)
ab78298 has been referenced in 14 publications.
- Wang J et al. Host Gene SEL1L Involved in Endoplasmic Reticulum-Associated Degradation Pathway Could Inhibit Hepatitis B Virus at RNA, DNA, and Protein Levels. Front Microbiol 10:2869 (2019). PubMed: 31921048
- Kitakaze K et al. Cell-based HTS identifies a chemical chaperone for preventing ER protein aggregation and proteotoxicity. Elife 8:N/A (2019). PubMed: 31843052
- Schultz ML et al. Coordinate regulation of mutant NPC1 degradation by selective ER autophagy and MARCH6-dependent ERAD. Nat Commun 9:3671 (2018). PubMed: 30202070
- Bhattacharya A et al. Hepatic Sel1L-Hrd1 ER-associated degradation (ERAD) manages FGF21 levels and systemic metabolism via CREBH. EMBO J 37:N/A (2018). PubMed: 30389665
- Glaeser K et al. ERAD-dependent control of the Wnt secretory factor Evi. EMBO J 37:N/A (2018). WB . PubMed: 29378775
Images
-
Western blot - Anti-SEL1L antibody (ab78298)Anti-SEL1L antibody (ab78298) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab78298 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753).
-
Western blot - Anti-SEL1L antibody (ab78298) This image is courtesy of an abreview submitted by Antibody Solutions Ltd.All lanes : Anti-SEL1L antibody (ab78298) at 1 µg/ml
Lane 1 : Mouse Pancreas
Lane 2 : Mouse Liver
Lane 3 : Mouse Heart
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Swine anti-Rabbit HRP at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 24& 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes