Anti-SCNN1B (phospho T615) antibody (ab79172)
Key features and details
- Rabbit polyclonal to SCNN1B (phospho T615)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-SCNN1B (phospho T615) antibody
See all SCNN1B primary antibodies -
Description
Rabbit polyclonal to SCNN1B (phospho T615) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat
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Immunogen
Synthesized phosphopeptide derived from human SCNN1B around the phosphorylation site of threonine 615 (P-G-TP-P-P).
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Positive control
- Human brain tissue; extracts from HeLa cells; COS7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SCNN1B (phospho T615) antibody (ab79172)All lanes : Anti-SCNN1B (phospho T615) antibody (ab79172) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from HeLa cells with immunizing phosphopeptide at 10 µg
Lysates/proteins at 10 µg per lane.
Predicted band size: 73 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCNN1B (phospho T615) antibody (ab79172)Immunohistochemiscal analysis of SCNN1B in paraffin embedded human brain tissue using a 1/50 dilution of ab79172 in the presence (right) or absence (left) of the immunizing phosphopeptide. -
Immunocytochemistry/ Immunofluorescence - Anti-SCNN1B (phospho T615) antibody (ab79172)Immunofluorescence analysis of SCNN1B in COS7 cells, using a 1/500 dilution of ab79172, in the presence (right) or absence (left) of the immunizing phosphopeptide.
