Anti-Salmonella LPS antibody [se-01] (ab243104)
![Anti-Salmonella LPS antibody [se-01] (ab243104)](https://www.abcam.com/ps/products/243/ab243104/Images/ab243104-332246-anti-salmonella-lps-antibody-se-01-western-blot.jpg "Anti-Salmonella LPS antibody [se-01] (ab243104)")
Key features and details
- Mouse monoclonal [se-01] to Salmonella LPS
- Suitable for: WB, ELISA
- Reacts with: Salmonella typhimurium
- Isotype: IgG1
Overview
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Product name
Anti-Salmonella LPS antibody [se-01] -
Description
Mouse monoclonal [se-01] to Salmonella LPS -
Host species
Mouse -
Specificity
Reacts with LPS of Salmonella enteritidis and Salmonella typhimurium. Does not react with other gram-negative food-poisoning bacteria like E. coli ,V. parahaemolyticus and Campylobacter species.
This antibody recognizes common antigenic determinants that are found in the structural components of Salmonella LPS.
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Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Salmonella typhimurium, Salmonella enteritidis -
Immunogen
Tissue, cells or virus corresponding to Salmonella LPS. Crude extract of Salmonella Enteritidis.
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Positive control
- WB: Crude extract and purified LPS of S. Enteritidis cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituents: PBS, 50% Glycerol
Filter sterilized -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
se-01 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Salmonella LPS antibody [se-01] (ab243104)All lanes : Anti-Salmonella LPS antibody [se-01] (ab243104) at 1/250 dilution
Lane 1 : Crude extract of S. enteritidis cells
Lane 2 : Purified LPS of S. enteritidis cells
Lysates/proteins at 1 µg per lane.Blocked with 5 % skimmed milk.
They showed similar band patterns characteristic of LPS, O-chain repeating unit.
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![ELISA - Anti-Salmonella LPS antibody [se-01] (ab243104)](https://www.abcam.com/ps/products/243/ab243104/Images/ab243104-332263-anti-salmonella-lps-antibody-se-01-elisa.jpg)
ELISA - Anti-Salmonella LPS antibody [se-01] (ab243104)Titration of LPS in the extract of S. Enteritidis cells by indirect ELISA.
The plate was coated with indicated amounts of the extract of S. Enteriditis cells. ab243104 was used at a 1/500 dilution.
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![Western blot - Anti-Salmonella LPS antibody [se-01] (ab243104)](https://www.abcam.com/ps/products/243/ab243104/Images/ab243104-332262-anti-salmonella-lps-antibody-se-01-western-blot.jpg)
Western blot - Anti-Salmonella LPS antibody [se-01] (ab243104)Reactivites of ab243104 with several food poisoning bacteria in Western Blot (Panel A) and Slot Blot (Panel B).
Lane 1: S. Enteritidis
Lane 2: V. Parahaemolyticus
Lane 3: E. Coli
Lane 4: E. Coli O157:H7
Panel B: Extracts of each strain of food poisoning bacteria were coated onto 5 areas of a nitrocellulose membrane. The membrane was soaked and reacted with ab243104.
SE: S. Enteritidis, EC: E. Coli, CJ: C, Jejuni, VP: V, Parahaemolyticus.
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![ELISA - Anti-Salmonella LPS antibody [se-01] (ab243104)](https://www.abcam.com/ps/products/243/ab243104/Images/ab243104-332247-anti-salmonella-lps-antibody-se-01-elisa.jpg)
ELISA - Anti-Salmonella LPS antibody [se-01] (ab243104)Titration of ab243104 by indirect ELISA using crude extract of S. Enteritidis.
The wells of the plate were coated with crude extract of S. Enteritidis (100 μL, 1 μg/mL). After blocking with 5% skimmed milk, 100 μl of antibody at the indicated dilution was added to the each well. HRP-conjugate goat anti-mouse IgG (100 μL, X2000 dilution) was added. Color was developed with orthophenylenediamine as substrate. Optical densities (OD) measured at 490nm.
