Anti-SA1 antibody (ab246988)
Key features and details
- Rabbit polyclonal to SA1
- Suitable for: IHC-P, WB, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SA1 antibody
See all SA1 primary antibodies -
Description
Rabbit polyclonal to SA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human SA1 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8WVM7 -
Positive control
- WB: U-138 MG, NIH/3T3, and NBT-II whole cell lysates; IHC-P: Human endometrium, fallopian tube, prostate, and small intestine tissues; ICC: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-SA1 antibody (ab246988)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SA1 (green) using ab246988 at 4 μg/ml in ICC.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody (ab246988)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labeling SA1 with ab246988 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-SA1 antibody (ab246988)All lanes : Anti-SA1 antibody (ab246988) at 0.4 µg/ml
Lane 1 : U-138 MG (Human glioblastoma) transfected with control siRNA, whole cell lysate
Lane 2 : U-138 MG transfected with target-specific siRNA #1, whole cell lysate
Lane 3 : U-138 MG transfected with target-specific siRNA #2, whole cell lysate
Predicted band size: 144 kDaRemaining relatve intensity is shown. Loading control: GAPDH.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody (ab246988)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fallopian tube tissue labeling SA1 with ab246988 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody (ab246988)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labeling SA1 with ab246988 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA1 antibody (ab246988)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labeling SA1 with ab246988 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-SA1 antibody (ab246988)All lanes : Anti-SA1 antibody (ab246988) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : NBT-II (rat Wistar bladder tumor cell line) whole cell lysate
Predicted band size: 144 kDa