Anti-RWDD1 antibody (ab241191)
Key features and details
- Rabbit polyclonal to RWDD1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RWDD1 antibody
See all RWDD1 primary antibodies -
Description
Rabbit polyclonal to RWDD1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human RWDD1 aa 193-243. The exact sequence is proprietary.
Database link: Q9H446 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-RWDD1 antibody (ab241191)
RWDD1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, prepared using NETN lysis buffer) using ab241191 at 8 µl per reaction. Western blot was performed on the immunoprecipitate using ab241191 at 1/1000 dilution.
Lane 1: ab241191 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Detection: Chemiluminescence with an exposure time of 30 seconds.
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Western blot - Anti-RWDD1 antibody (ab241191)All lanes : Anti-RWDD1 antibody (ab241191) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutesLysate prepared using NETN lysis buffer.
