Anti-RPL5 antibody (ab137617)
Key features and details
- Rabbit polyclonal to RPL5
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RPL5 antibody
See all RPL5 primary antibodies -
Description
Rabbit polyclonal to RPL5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Cow, Pig
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 142-297 of Human RPL5.
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji cell lysates; Mouse brain; HepG2 cells; Gastric CA N87 xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RPL5 antibody (ab137617)All lanes : Anti-RPL5 antibody (ab137617) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HeLaS3 whole cell lysate
Lane 4 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 34 kDa
12% SDS PAGE -
Western blot - Anti-RPL5 antibody (ab137617)Anti-RPL5 antibody (ab137617) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 34 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-RPL5 antibody (ab137617)ab137617 at 1/500 dilution staining RPL5 in 4% paraformaldehyde (room temperature for 15 min) fixed HepG2 cells by Immunofluorescence (green). Blue staining: Hoechst 33343. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL5 antibody (ab137617)ab137617 at 1/100 dilution, staining RPL5 in paraffin embedded Gastric CA N87 xenograft tissue by Immunohistochemistry.
