Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RP105 antibody [EPR14720] (ab184956)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14720] to RP105
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-RP105 antibody [EPR14720]
See all RP105 primary antibodies
Description

Rabbit monoclonal [EPR14720] to RP105
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Ramos, human lymph node, Daudi lysates, human spleen tissue, Ramos cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14720
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody [EPR14720] (ab184956)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling RP105 with ab184956 at 1/500 dilution and HRP Polymer to Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-RP105 antibody [EPR14720] (ab184956)

All lanes : Anti-RP105 antibody [EPR14720] (ab184956) at 1/2000 dilution

Lane 1 : Human lymph node lysate
Lane 2 : Ramos cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 74 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Western blot - Anti-RP105 antibody [EPR14720] (ab184956)

Anti-RP105 antibody [EPR14720] (ab184956) at 1/2000 dilution + Daudi cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 74 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-RP105 antibody [EPR14720] (ab184956)

Immunocytochemical analysis of Ramos cells labeling RP105 with ab184956 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution. Counterstained with DAPI. Cells fixation: -20℃ Acetone.
Anti-RP105 antibody [EPR14720] (ab184956)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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