Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RP105 antibody [EPR14720] (ab184956)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14720] to RP105
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-RP105 antibody [EPR14720]
    See all RP105 primary antibodies

  • Description

    Rabbit monoclonal [EPR14720] to RP105

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Ramos, human lymph node, Daudi lysates, human spleen tissue, Ramos cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody [EPR14720] (ab184956)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RP105 antibody [EPR14720] (ab184956)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling RP105 with ab184956 at 1/500 dilution and HRP Polymer to Rabbit IgG.  Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-RP105 antibody [EPR14720] (ab184956)
    Western blot - Anti-RP105 antibody [EPR14720] (ab184956)
    All lanes : Anti-RP105 antibody [EPR14720] (ab184956) at 1/2000 dilution

    Lane 1 : Human lymph node lysate
    Lane 2 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 74 kDa
    Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.

  • Western blot - Anti-RP105 antibody [EPR14720] (ab184956)
    Western blot - Anti-RP105 antibody [EPR14720] (ab184956)
    Anti-RP105 antibody [EPR14720] (ab184956) at 1/2000 dilution + Daudi cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 74 kDa
    Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-RP105 antibody [EPR14720] (ab184956)
    Immunocytochemistry/ Immunofluorescence - Anti-RP105 antibody [EPR14720] (ab184956)

    Immunocytochemical analysis of Ramos cells labeling RP105 with ab184956 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution. Counterstained with DAPI. Cells fixation: -20℃ Acetone.

  • Anti-RP105 antibody [EPR14720] (ab184956)
    Anti-RP105 antibody [EPR14720] (ab184956)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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