Anti-RNF40 antibody (ab26082)
Key features and details
- Rabbit polyclonal to RNF40
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF40 antibody
See all RNF40 primary antibodies -
Description
Rabbit polyclonal to RNF40 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human RNF40.
Read Abcam's proprietary immunogen policy (Peptide available as ab27760.) -
Positive control
- This antibody gave a positive signal in HeLa nuclear extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 114 kDa). Abcam recommends using 5% milk as the blocking agent.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 114 kDa). Abcam recommends using 5% milk as the blocking agent.Target
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Function
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). Forms a ubiquitin ligase complex in cooperation with the E2 enzyme UBE2E1/UBCH6. It thereby plays a central role in histone code and gene regulation. Required for transcriptional activation of Hox genes. -
Tissue specificity
Ubiquitously expressed. Expressed at higher level in testis, heart and pancreas, while it is only weakly expressed in lung, skeletal muscle and small intestine. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the BRE1 family.
Contains 1 RING-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9810 Human
- Omim: 607700 Human
- SwissProt: O75150 Human
- Unigene: 65238 Human
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Alternative names
- 95 kDa retinoblastoma associated protein antibody
- 95 kDa retinoblastoma protein binding protein antibody
- 95 kDa retinoblastoma-associated protein antibody
see all
Images
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Western blot - Anti-RNF40 antibody (ab26082)Anti-RNF40 antibody (ab26082) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 35 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends using 5% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (3)
ab26082 has been referenced in 3 publications.
- Long L et al. The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination. J Biol Chem 289:8916-30 (2014). WB . PubMed: 24526689
- Lilley CE et al. A viral E3 ligase targets RNF8 and RNF168 to control histone ubiquitination and DNA damage responses. EMBO J 29:943-55 (2010). WB ; Human . PubMed: 20075863
- Pirngruber J et al. CDK9 directs H2B monoubiquitination and controls replication-dependent histone mRNA 3'-end processing. EMBO Rep 10:894-900 (2009). WB ; Human . PubMed: 19575011
Images
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Western blot - Anti-RNF40 antibody (ab26082)Anti-RNF40 antibody (ab26082) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 35 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Abcam recommends using 5% milk as the blocking agent. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
