Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RNF128/GRAIL antibody [EPR7426(2)] - BSA and Azide free (ab248811)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7426(2)] to RNF128/GRAIL - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RNF128/GRAIL antibody [EPR7426(2)] - BSA and Azide free
    See all RNF128/GRAIL primary antibodies

  • Description

    Rabbit monoclonal [EPR7426(2)] to RNF128/GRAIL - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248811 is the carrier-free version of ab137088. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248811 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] - BSA and Azide free (ab248811)
    Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] - BSA and Azide free (ab248811)
    All lanes : Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088) at 1/1000 dilution (purified)

    Lane 1 : RAW 264.7 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab137088, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] - BSA and Azide free (ab248811)
    Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] - BSA and Azide free (ab248811)
    All lanes : Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088) at 1/1000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 46 kDa why is the actual band size different from the predicted?



    This data was developed using ab137088, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] - BSA and Azide free (ab248811)
    Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] - BSA and Azide free (ab248811)
    All lanes : Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : 293T cell lysates
    Lane 3 : U-87 MG cell lysates
    Lane 4 : RAW 264.7 cell lysates
    Lane 5 : NIH 3T3 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 47 kDa



    This data was developed using ab137088, the same antibody clone in a different buffer formulation.

  • Anti-RNF128/GRAIL antibody [EPR7426(2)] - BSA and Azide free (ab248811)
    Anti-RNF128/GRAIL antibody [EPR7426(2)] - BSA and Azide free (ab248811)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.