Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7426(2)] to RNF128/GRAIL
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RNF128/GRAIL antibody [EPR7426(2)]
    See all RNF128/GRAIL primary antibodies

  • Description

    Rabbit monoclonal [EPR7426(2)] to RNF128/GRAIL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human RNF128/GRAIL aa 350 to the C-terminus.
    Database link: Q8TEB7

  • Positive control

    • WB: HeLa, 293T, U-87 MG, RAW 264.7 and NIH 3T3 cell lysates

  • General notes

     

     

    Previously labelled as RNF128. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7426(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)
    Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)
    All lanes : Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088) at 1/1000 dilution (purified)

    Lane 1 : RAW264.7 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)
    Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)
    All lanes : Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088) at 1/1000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 47 kDa
    Observed band size: 46 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)
    Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)
    All lanes : Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088) at 1/1000 dilution

    Lane 1 : HeLa cell lysates
    Lane 2 : 293T cell lysates
    Lane 3 : U-87 MG cell lysates
    Lane 4 : RAW 264.7 cell lysates
    Lane 5 : NIH 3T3 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 47 kDa

  • Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)
    Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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