Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 16, 2021

Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7426(2)] to RNF128/GRAIL
Suitable for: WB
Reacts with: Mouse, Human

Product name

Anti-RNF128/GRAIL antibody [EPR7426(2)]
See all RNF128/GRAIL primary antibodies
Description

Rabbit monoclonal [EPR7426(2)] to RNF128/GRAIL
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

Human

See all applications and species data
Immunogen

corresponding to Human RNF128/GRAIL aa 350 to the C-terminus.
Database link: Q8TEB7
Positive control
- WB: HeLa, 293T, U-87 MG, RAW 264.7 and NIH 3T3 cell lysates
General notes
Previously labelled as RNF128.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7426(2)
Isotype

IgG
Research areas

Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)

All lanes : Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088) at 1/1000 dilution (purified)

Lane 1 : RAW264.7 cell lysate
Lane 2 : NIH/3T3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 46 kDa
why is the actual band size different from the predicted?
Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)

All lanes : Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088) at 1/1000 dilution (purified)

Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : U87-MG cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)

All lanes : Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088) at 1/1000 dilution

Lane 1 : HeLa cell lysates
Lane 2 : 293T cell lysates
Lane 3 : U-87 MG cell lysates
Lane 4 : RAW 264.7 cell lysates
Lane 5 : NIH 3T3 cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47 kDa
Anti-RNF128/GRAIL antibody [EPR7426(2)] (ab137088)

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