Anti-Ribophorin I antibody [EPR17044(B)] - BSA and Azide free (ab251245)
![Anti-Ribophorin I antibody [EPR17044(B)] - BSA and Azide free (ab251245)](https://www.abcam.com/ps/products/251/ab251245/Images/ab251245-403475-anti-ribophorin-i-antibody-epr17044-b-bsa-and-azide-free-western-blot.jpg "Anti-Ribophorin I antibody [EPR17044(B)] - BSA and Azide free (ab251245)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17044(B)] to Ribophorin I - BSA and Azide free
- Suitable for: WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-Ribophorin I antibody [EPR17044(B)] - BSA and Azide free
See all Ribophorin I primary antibodies -
Description
Rabbit monoclonal [EPR17044(B)] to Ribophorin I - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251245 is the carrier-free version of ab197888. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251245 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR17044(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ribophorin I antibody [EPR17044(B)] - BSA and Azide free (ab251245)All lanes : Anti-Ribophorin I antibody [EPR17044(B)] (ab197888) at 1/20000 dilution
Lane 1 : BxPC-3 (human Pancreas adenocarcinoma ) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 69 kDa
Exposure time: 1 minuteThis data was developed using ab197888, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Ribophorin I antibody [EPR17044(B)] - BSA and Azide free (ab251245)](https://www.abcam.com/ps/products/251/ab251245/Images/ab251245-399102-anti-ribophorin-i-antibody-epr17044-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Ribophorin I antibody [EPR17044(B)] - BSA and Azide free (ab251245)All lanes : Anti-Ribophorin I antibody [EPR17044(B)] (ab197888) at 1/2000 dilution
Lane 1 : Rat kidney lysate
Lane 2 : Rat spleen lysate
Lane 3 : C6 (Rat glial tumor cells) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 69 kDa
Exposure time: 3 minutesThis data was developed using ab197888, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-Ribophorin I antibody [EPR17044(B)] - BSA and Azide free (ab251245)](https://www.abcam.com/ps/products/251/ab251245/Images/ab251245-3-benefits-of-recombinant-antibodies.png)
Anti-Ribophorin I antibody [EPR17044(B)] - BSA and Azide free (ab251245)