Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2020(N)] to Retinoic Acid Receptor gamma - N-terminal
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal
    See all Retinoic Acid Receptor gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR2020(N)] to Retinoic Acid Receptor gamma - N-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • NCCIT, NTERA2/D1 and HeLa cell lysates; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
    Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
    All lanes : Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : RARG (Acid Receptor gamma) knockout HAP1 whole cell lysate
    Lane 3 : A375 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab187159 observed at 50 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab187159 was shown to recognize Acid Receptor gamma in wild-type HAP1 cells as signal was lost at the expected MW in RARG (Acid Receptor gamma) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and RARG (Acid Receptor gamma) knockout samples were subjected to SDS-PAGE. Ab187159 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
    Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
    All lanes : Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution

    Lane 1 : NCCIT cell lysate
    Lane 2 : NTERA2/D1 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
    Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)

    Immunofluorescent analysis of HeLa cells labeling Retinoic Acid Receptor gamma with ab187159 at 1/250 and DAPI staining (blue).

  • Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
    Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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