Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
![Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)](https://www.abcam.com/ps/products/187/ab187159/Images/ab187159-310165-anti-retinoic-acid-receptor-gamma-antibody-epr2020n-n-terminal-western-blot.jpg "Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2020(N)] to Retinoic Acid Receptor gamma - N-terminal
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal
See all Retinoic Acid Receptor gamma primary antibodies -
Description
Rabbit monoclonal [EPR2020(N)] to Retinoic Acid Receptor gamma - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- NCCIT, NTERA2/D1 and HeLa cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2020(N) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)All lanes : Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : RARG (Acid Receptor gamma) knockout HAP1 whole cell lysate
Lane 3 : A375 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa
Observed band size: 50 kDaLanes 1 - 3: Merged signal (red and green). Green - ab187159 observed at 50 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab187159 was shown to recognize Acid Receptor gamma in wild-type HAP1 cells as signal was lost at the expected MW in RARG (Acid Receptor gamma) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and RARG (Acid Receptor gamma) knockout samples were subjected to SDS-PAGE. Ab187159 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)](https://www.abcam.com/ps/products/187/ab187159/Images/ab187159-220344-anti-retinoic-acid-receptor-gamma-antibody-epr2020n-n-terminal-western-blot.jpg)
Western blot - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)All lanes : Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159) at 1/1000 dilution
Lane 1 : NCCIT cell lysate
Lane 2 : NTERA2/D1 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 50 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)](https://www.abcam.com/ps/products/187/ab187159/Images/ab187159-220346-anti-retinoic-acid-receptor-gamma-antibody-epr2020n-n-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)Immunofluorescent analysis of HeLa cells labeling Retinoic Acid Receptor gamma with ab187159 at 1/250 and DAPI staining (blue).
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![Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)](https://www.abcam.com/ps/products/187/ab187159/Images/ab187159-4-benefits-of-recombinant-antibodies.png)
Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (ab187159)
