Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824)

Key features and details

  • Rat monoclonal [ER-TR7] to Reticular Fibroblasts and Reticular Fibres
  • Suitable for: Flow Cyt, IHC-Fr
  • Reacts with: Mouse, Human, Pig
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7]

  • Description

    Rat monoclonal [ER-TR7] to Reticular Fibroblasts and Reticular Fibres

  • Host species

    Rat

  • Tested applications

    Suitable for: Flow Cyt, IHC-Frmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Human, Pig

  • Immunogen

    Murine thymic reticulum

  • Epitope

    The antigen has not been not fully characterized. The recognized epitope may be part of reticulin.

  • Positive control

    • Mouse spleen, reticular fibroblasts and reticular fibres, Human tonsil.

  • General notes

    Antigen Distribution: Isolated cells: The antigen is found in the cytoplasm of fibroblasts and fibroblast cell lines. Tissue sections: In all organs tested so far, the antigen is expressed in connective tissues which form a supporting network between parenchymal cells (see table below). Thus, the supportive mesenchymal structures of larger vessels can be studied. In spleen, a very clear delineation of red and white pulp is obtained. Capsule, sinuses, follicules, paracortex and medullary cords are also clearly delineated in lymph nodes.


    This monoclonal antibody [ER-TR7] detects an antigen present in, and produced by, reticular fibroblasts. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

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Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.5% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Primary antibody notes

    This monoclonal antibody [ER-TR7] detects an antigen present in, and produced by, reticular fibroblasts. The antibody is useful to stain the microanatomy of various organs, in particular the connective tissue framework in lymphoid organs. The antibody also stains subendothelial deposits in the plaque area of atherosclerotic plaques.

  • Clonality

    Monoclonal

  • Clone number

    ER-TR7

  • Isotype

    IgG2a

  • Research areas

Images

  • Immunohistochemistry (Frozen sections) - Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824)
    Immunohistochemistry (Frozen sections) - Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824)

    Immunohistochemical analysis of Mouse liver frozen section, labeling reticular fibres with ab51824.

  • Immunohistochemistry (Frozen sections) - Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824)
    Immunohistochemistry (Frozen sections) - Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824) This image is courtesy of an Abreview by Lynda Coughlan.
    ab51824 staining Reticular Fibroblasts and Reticular Fibres in Mouse spleen tissue sections by Immunohistochemistry (Frozen sections). The sections were fixed with 4% PFA for 10 minutes at room temperature prior to blocking with 10% Goat serum for 30 minutes at 15°C. The primary antibody was diluted 1/500 in PBS with 2% Goat serum and incubated with the sample for 1 hour at 15°C. An Alexa Fluor® 488-conjugated Goat anti-Rat IgG polyclonal was used as the secondary antibody, diluted 1/500.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824)
    Immunohistochemistry (Frozen sections) - Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824)
    ab51824 at 1 &microg/ml staining mouse spleen; frozen section.
  • Flow Cytometry - Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824)
    Flow Cytometry - Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824)
    Overlay histogram showing HT1080 cells stained with ab51824 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51824 , 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758](ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunohistochemistry (Frozen sections) - Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824)
    Immunohistochemistry (Frozen sections) - Anti-Reticular Fibroblasts and Reticular Fibres antibody [ER-TR7] (ab51824)

    Immunohistochemical analysis of Human skin frozen section, labeling reticular fibres with ab51824.

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