Anti-RECQ1 antibody (ab272855)
Key features and details
- Rabbit polyclonal to RECQ1
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RECQ1 antibody
See all RECQ1 primary antibodies -
Description
Rabbit polyclonal to RECQ1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human RECQ1 (N terminal). The exact sequence is proprietary.
Database link: P46063 -
Positive control
- WB: A431, HeLa and A375 whole cell lysate. IHC-P: Human breast carcinoma tissue. ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RECQ1 antibody (ab272855)All lanes : Anti-RECQ1 antibody (ab272855) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : A-375 (human malignant melanoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.7.5% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RECQ1 antibody (ab272855)Paraffin-embedded human breast carcinoma tissue stained for RECQ1 using ab272855 at 1/250 dilution in immunohistochemical analysis.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15 mins. -
Immunocytochemistry/ Immunofluorescence - Anti-RECQ1 antibody (ab272855)Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RECQ1 (green) using ab272855 at 1/500 dilution.
Counterstain: Phalloidin, a cytoskeleton marker (red).
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Immunoprecipitation - Anti-RECQ1 antibody (ab272855)Immunoprecipitation of RECQ1 protein from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab272855 at 5 μg. ab272855 also used for WB followed by an anti-Rabbit IgG-HRP secondary.
Lane 1: Control IgG.
Lane 2: ab272855 IP in HeLa whole cell lysate.