Antibodies, Proteins, Kits and Reagents for Life Science

Anti-RDH5 antibody [EPR15191] - BSA and Azide free (ab251299)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15191] to RDH5 - BSA and Azide free
Suitable for: WB, IP, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-RDH5 antibody [EPR15191] - BSA and Azide free
See all RDH5 primary antibodies
Description

Rabbit monoclonal [EPR15191] to RDH5 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IP, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
ab251299 is the carrier-free version of ab200197.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15191
Isotype

IgG
Research areas

Western blot - Anti-RDH5 antibody [EPR15191] - BSA and Azide free (ab251299)

All lanes : Anti-RDH5 antibody [EPR15191] (ab200197) at 1/5000 dilution

Lane 1 : Mouse eyeball tissue lysate
Lane 2 : Rat eyeball tissue lysate
Lane 3 : Mouse spleen tissue lysate
Lane 4 : Rat spleen tissue lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) cell lysate
Lane 6 : NIH/3T3 (Mouse embyro fibroblast cells) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Exposure time: 15 seconds

This data was developed using ab200197, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-RDH5 antibody [EPR15191] - BSA and Azide free (ab251299)

Anti-RDH5 antibody [EPR15191] (ab200197) at 1/200 dilution + Y79 (Human retinoblastoma cell line) cell lysate at 30 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa
Observed band size: 35 kDa

Exposure time: 3 minutes

This data was developed using ab200197, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry - Anti-RDH5 antibody [EPR15191] - BSA and Azide free (ab251299)

This data was developed using ab200197, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Y79 (Human retinoblastoma cell line) cells labeling RDH5 with ab200197 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Cytoplasm staining on Y79 cell line is observed.The nuclear counterstain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-
-ve control 1: ab200197 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Immunoprecipitation - Anti-RDH5 antibody [EPR15191] - BSA and Azide free (ab251299)

This data was developed using ab200197, the same antibody clone in a different buffer formulation.RDH5 was immunoprecipitated from 1mg of Rat eyeball whole cell lysate with ab200197 at 1/30 dilution. Lane 1: Rat eyeball whole cell lysate 10ug (Input).Lane 2: ab200197 IP in Rat eyeball whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200197 in Rat eyeball whole cell lysate.Western blot was performed from the immunoprecipitate using ab200197 at 1/1000 dilution.Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds.
Anti-RDH5 antibody [EPR15191] - BSA and Azide free (ab251299)

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