Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RCL antibody [EPR8685] - BSA and Azide free (ab248977)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8685] to RCL - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-RCL antibody [EPR8685] - BSA and Azide free
    See all RCL primary antibodies

  • Description

    Rabbit monoclonal [EPR8685] to RCL - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248977 is the carrier-free version of ab150413. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248977 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RCL antibody [EPR8685] - BSA and Azide free (ab248977)
    Western blot - Anti-RCL antibody [EPR8685] - BSA and Azide free (ab248977)
    All lanes : Anti-RCL antibody [EPR8685] (ab150413) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 19 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab150413, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCL antibody [EPR8685] - BSA and Azide free (ab248977)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCL antibody [EPR8685] - BSA and Azide free (ab248977)
    This data was developed using ab150413, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RCL with ab150413 antibody at a dilution of 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCL antibody [EPR8685] - BSA and Azide free (ab248977)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCL antibody [EPR8685] - BSA and Azide free (ab248977)
    This data was developed using ab150413, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling RCL with ab150413 antibody at a dilution of 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-RCL antibody [EPR8685] - BSA and Azide free (ab248977)
    Anti-RCL antibody [EPR8685] - BSA and Azide free (ab248977)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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