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Cell Biology Other Antibodies

Anti-RCL antibody [EPR8685] (ab150413)

Price and availability

298 185 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-RCL antibody [EPR8685] (ab150413)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8685] to RCL
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-RCL antibody [EPR8685]
    See all RCL primary antibodies
  • Description

    Rabbit monoclonal [EPR8685] to RCL
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human RCL. The exact sequence is proprietary.

  • Positive control

    • K562, Jurkat, HepG2 and 293T cell lysates, Human breast carcinoma and Human kidney tissues
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8685
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies

Images

  • Western blot - Anti-RCL antibody [EPR8685] (ab150413)
    Western blot - Anti-RCL antibody [EPR8685] (ab150413)
    All lanes : Anti-RCL antibody [EPR8685] (ab150413) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 19 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCL antibody [EPR8685] (ab150413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCL antibody [EPR8685] (ab150413)

    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RCL with ab150413 antibody at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCL antibody [EPR8685] (ab150413)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCL antibody [EPR8685] (ab150413)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling RCL with ab150413 antibody at a dilution of 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-RCL antibody [EPR8685] (ab150413)
    Anti-RCL antibody [EPR8685] (ab150413)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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