Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6850(B)] to RBX1 - BSA and Azide free
  • Suitable for: IHC-P, IP, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free
    See all RBX1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6850(B)] to RBX1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248556 is the carrier-free version of ab133565. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248556 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)
    Western blot - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)
    All lanes : Anti-RBX1 antibody [EPR6850(B)] (ab133565) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 12 kDa



    This data was developed using ab133565, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)
    This data was developed using ab133565, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling RBX1 with ab133565 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)
    Flow Cytometry - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)
    This data was developed using ab133565, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab133565 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133565, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunoprecipitation - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)
    Immunoprecipitation - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)

    This data was developed using ab133565, the same antibody clone in a different buffer formulation.

    ab133565 at 1/70 immunoprecipitating RBX1 in HepG2 whole cell lysate.

    For western blotting, a primary antibody was used at 1/1000 and a HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/1000).

    Blocking and dilution buffer: 5% NFDM/TBST.

  • OI-RD Scanning - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)
    OI-RD Scanning - Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)
    This data was developed using ab133565, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)
    Anti-RBX1 antibody [EPR6850(B)] - BSA and Azide free (ab248556)

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