Anti-RbBP5 antibody (ab80074)
Key features and details
- Rabbit polyclonal to RbBP5
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RbBP5 antibody
See all RbBP5 primary antibodies -
Description
Rabbit polyclonal to RbBP5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human RbBP5 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab87828) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: WERI; Y79; SW480; HEK293.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications CowZebrafishApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).Target
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Function
As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Contains 6 WD repeats. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5929 Human
- Omim: 600697 Human
- SwissProt: Q15291 Human
- Unigene: 519230 Human
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Alternative names
- RBBP 5 antibody
- RBBP-5 antibody
- RBBP5 antibody
see all
Images
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Western blot - Anti-RbBP5 antibody (ab80074)All lanes : Anti-RbBP5 antibody (ab80074) at 1 µg/ml
Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 60,65 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
RbBP5 contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed. The antibody should cross-react with both isoform 1 (59-kDa) and isoform 2 (55-kDa) of RbBP5.
Datasheets and documents
References (1)
ab80074 has been referenced in 1 publication.
- Fang L et al. SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis. Cancer Cell 34:103-118.e9 (2018). PubMed: 30008322
Images
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Western blot - Anti-RbBP5 antibody (ab80074)All lanes : Anti-RbBP5 antibody (ab80074) at 1 µg/ml
Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 60,65 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
RbBP5 contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed. The antibody should cross-react with both isoform 1 (59-kDa) and isoform 2 (55-kDa) of RbBP5.
