Anti-RARRES1 antibody (ab76530)
Key features and details
- Rabbit polyclonal to RARRES1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RARRES1 antibody
See all RARRES1 primary antibodies -
Description
Rabbit polyclonal to RARRES1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to RARRES1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab87448) -
Positive control
- Recombinant Human RARRES1 protein (ab114592) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Human Liver Tissue; Human Kidney Tissue; HepG2 Whole Cell; HEK293 Whole Cell
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).Target
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Sequence similarities
Belongs to the protease inhibitor I47 (latexin) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 5918 Human
- Omim: 605090 Human
- SwissProt: P49788 Human
- Unigene: 131269 Human
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Alternative names
- 5430417P09Rik antibody
- AI662122 antibody
- RAR-responsive protein TIG1 antibody
see all
Images
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Western blot - Anti-RARRES1 antibody (ab76530)All lanes : Anti-RARRES1 antibody (ab76530) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 62 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
RARRES1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (2)
ab76530 has been referenced in 2 publications.
- Pang B et al. Landscape of tumor suppressor long noncoding RNAs in breast cancer. J Exp Clin Cancer Res 38:79 (2019). PubMed: 30764831
- Chen HL et al. Secretome survey of human plexiform neurofibroma derived Schwann Cells reveals a secreted form of the RARRES1 protein. Int J Mol Sci 13:9380-99 (2012). PubMed: 22942771
Images
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Western blot - Anti-RARRES1 antibody (ab76530)All lanes : Anti-RARRES1 antibody (ab76530) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 62 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
RARRES1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
