Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Raptor antibody [EP539Y] (ab40768)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP539Y] to Raptor
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Raptor antibody [EP539Y]
    See all Raptor primary antibodies

  • Description

    Rabbit monoclonal [EP539Y] to Raptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Raptor (C terminal). The exact sequence is proprietary.

  • Positive control

    • Normal human colon and Human cardiac muscle; mouse and rat brain tissue lysate HEK-293, HeLa, MCF-7 and A431 cell lysate;

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
    Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
    All lanes : Anti-Raptor antibody [EP539Y] (ab40768) at 1/5000 dilution

    Lane 1 : HEK-293 (human embryonic kidney) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 3 : MCF-7 (human breast carcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 149 kDa
    Observed band size: 140 kDa
    why is the actual band size different from the predicted?



    Blocking/Diluting buffer 5% NFDM /TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling Raptor with purified ab40768 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

  • Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
    Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
    Anti-Raptor antibody [EP539Y] (ab40768) at 1/5000 dilution + Rat brain tissue lysate at 20 mg/ml

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 149 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer 5% NFDM /TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (ab40768)
    Immunohistochemical analysis of paraffin-embedded human colon using anti-Raptor ab40768 at 1/50 dilution.
  • Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
    Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
    Anti-Raptor antibody [EP539Y] (ab40768) at 1/1000 dilution + Mouse brain tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 149 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer 5% NFDM /TBST
  • Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
    Western blot - Anti-Raptor antibody [EP539Y] (ab40768)
    All lanes : Anti-Raptor antibody [EP539Y] (ab40768) at 1/500 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 149 kDa
    Observed band size: 140 kDa why is the actual band size different from the predicted?
    Additional bands at: 21 kDa, 37 kDa. We are unsure as to the identity of these extra bands.

  • Anti-Raptor antibody [EP539Y] (ab40768)
    Anti-Raptor antibody [EP539Y] (ab40768)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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