Anti-RanBP1 antibody (ab264129)
Key features and details
- Rabbit polyclonal to RanBP1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RanBP1 antibody
See all RanBP1 primary antibodies -
Description
Rabbit polyclonal to RanBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human RanBP1 aa 1-50. The exact sequence is proprietary.
Database link: P43487 -
Positive control
- WB: HeLa and HEK-293T whole cell lysate. IHC-P: Human colon carcinoma tissue. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RanBP1 antibody (ab264129)All lanes : Anti-RanBP1 antibody (ab264129) at 0.4 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Exposure time: 3 minutes4-20% SDS-PAGE.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RanBP1 antibody (ab264129)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling RanBP1 with ab264129 at 1/5,000 dilution. Detection: DAB.
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Immunoprecipitation - Anti-RanBP1 antibody (ab264129)RanBP1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264129 at 3µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264129 at 1µg/ml.
Lane 1: ab264129 IP in HeLa whole cell lysate, lot 1.
Lane 2: ab264129 IP in HeLa whole cell lysate, lot 2.
Lane 3: Control IgG.Exposure time: 3 mins.
