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Neuroscience Neurology process Neurogenesis

Anti-RAI1 antibody (ab86599)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-RAI1 antibody (ab86599)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to RAI1
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RAI1 antibody
    See all RAI1 primary antibodies
  • Description

    Rabbit polyclonal to RAI1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 25 and 75 of Human RAI1 (NP_109590.3).

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurogenesis

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAI1 antibody (ab86599)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAI1 antibody (ab86599)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling RAI1 with ab86599 at 1/1000 (1µg/ml). Detection: DAB.
  • Western blot - Anti-RAI1 antibody (ab86599)
    Western blot - Anti-RAI1 antibody (ab86599)
    All lanes : Anti-RAI1 antibody (ab86599) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique.

    Predicted band size: 203 kDa
    Observed band size: 280 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-RAI1 antibody (ab86599)
    Immunoprecipitation - Anti-RAI1 antibody (ab86599)
    Western Blot detection of RAI1 in Immunoprecipitates of Whole cell lysate of HeLa cells (1 mg for IP, 20% of IP loaded) using ab86599 at 10 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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