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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family

Anti-RAB27A + RAB27B antibody - C-terminal (ab192673)

Anti-RAB27A + RAB27B antibody - C-terminal (ab192673)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Goat polyclonal to RAB27A + RAB27B - C-terminal
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RAB27A + RAB27B antibody - C-terminal
  • Description

    Goat polyclonal to RAB27A + RAB27B - C-terminal
  • Host species

    Goat
  • Specificity

    This antibody detects total RAB27A + RAB27B levels
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment within Mouse RAB27A + RAB27B aa 100 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. RAB27A, produced in E coli
    Database link: Q9ERI2

    Recombinant fragment within Mouse RAB27A + RAB27B aa 100 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. RAB27B, produced in E coli
    Database link: Q99P58

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Melan-Ink; AtT 20 cell lysates; B65-RPE07 cells; primary RPE cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 79% PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family

Images

  • Western blot - Anti-RAB27A + RAB27B antibody - C-terminal (ab192673)
    Western blot - Anti-RAB27A + RAB27B antibody - C-terminal (ab192673)
    All lanes : Anti-RAB27A + RAB27B antibody - C-terminal (ab192673) at 1/500 dilution

    Lane 1 : Mela-Ink cell lysate
    Lane 2 : AtT 20 cell lysate

    Secondary
    All lanes : HRP conjugated rabbit anti goat at 1/10000 dilution

    Predicted band size: 25, 24 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB27A + RAB27B antibody - C-terminal (ab192673)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB27A + RAB27B antibody - C-terminal (ab192673)

    Immunofluorescence analysis of primary RPE cells labeling RAB27A + RAB27B using ab192673 at 1/50 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB27A + RAB27B antibody - C-terminal (ab192673)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB27A + RAB27B antibody - C-terminal (ab192673)

    Immunofluorescence analysis of B6-RPE07 cells labeling RAB27A + RAB27B using ab192673 at 1/50 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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