Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (ab249322)
![Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (ab249322)](https://www.abcam.com/ps/products/249/ab249322/Images/ab249322-405777-anti-ptdss1-antibody-epr8118-2-bsa-and-azide-free-western-blot.jpg "Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (ab249322)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8118(2)] to PTDSS1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free
See all PTDSS1 primary antibodies -
Description
Rabbit monoclonal [EPR8118(2)] to PTDSS1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249322 is the carrier-free version of ab157222. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249322 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8118(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (ab249322)All lanes : Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lane 4 : Human fetal brain tissue lysate
Lane 5 : A549 cell lysate
Lane 6 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 56 kDaThis data was developed using ab157222, the same antibody clone in a different buffer formulation.
The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and 10938271).
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![Western blot - Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (ab249322)](https://www.abcam.com/ps/products/249/ab249322/Images/ab249322-403364-anti-ptdss1-antibody-epr8118-2-bsa-and-azide-free-western-blot-human.jpg)
Western blot - Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (ab249322)Anti-PTDSS1 antibody [EPR8118(2)] (ab157222) at 1/1000 dilution + His-tagged human PTDSS1 (aa2-35-GGGGS-aa2-35-GGGGS-aa2-35) recombinant protein 15ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 56 kDa
Exposure time: 1 secondThis data was developed using ab157222, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Observed band size: 17 kDa (Recombinant protein fragment).
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![Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (ab249322)](https://www.abcam.com/ps/products/249/ab249322/Images/ab249322-1-anti-ptdss1-antibody-epr8118-2-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (ab249322)This data was developed using ab157222, the same antibody clone in a different buffer formulation.Immunoprecipitation : 293T cell lysate immunoprecipitated with ab157222 at 1/10 Western Blot: ab157222 at 1/1000 labeling PTDSS1 in immunoprecipitation pellet from 293T cell lysate Secondary Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution Predicted band size : 56 kDa The observed band size of ab157222 is 42kda, this is attributed to the high content of hydrophobic amino acids in PSS1 (PMID: 8898108 and PMID: 10938271). -
![Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (ab249322)](https://www.abcam.com/ps/products/249/ab249322/Images/ab249322-4-benefits-of-recombinant-antibodies.png)
Anti-PTDSS1 antibody [EPR8118(2)] - BSA and Azide free (ab249322)