Anti-Protein Z antibody (ab85317)
Key features and details
- Rabbit polyclonal to Protein Z
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Protein Z antibody
See all Protein Z primary antibodies -
Description
Rabbit polyclonal to Protein Z -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Protein Z aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab129946) -
Positive control
- This antibody gave a positive signal in HepG2 whole cell lysate as well as the following tissue lysates: Human Heart; Human Spleen; Human Bone Tumour; Human Bone Marrow. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: DU145.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanAll applications ChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).Notes ICC/IF
Use a concentration of 5 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa).Target
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Function
Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. -
Tissue specificity
Plasma. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 8858 Human
- Omim: 176895 Human
- SwissProt: P22891 Human
- Unigene: 1011 Human
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Alternative names
- Protein Z, vitamin K dependent plasma glycoprotein antibody
- PROZ antibody
- PROZ_HUMAN antibody
see all
Images
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Western blot - Anti-Protein Z antibody (ab85317)All lanes : Anti-Protein Z antibody (ab85317) at 1 µg/ml (Milk Blocking - 5%)
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human bone tumor tissue lysate - total protein (ab29359)
Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa, 55 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 42 kDa could potentially be a cleaved form of Protein Z due to the presemce of a 23 amino acid signal peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Protein Z antibody (ab85317)ICC/IF image of ab85317 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85317 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (0)
ab85317 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Protein Z antibody (ab85317)All lanes : Anti-Protein Z antibody (ab85317) at 1 µg/ml (Milk Blocking - 5%)
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : Human bone tumor tissue lysate - total protein (ab29359)
Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa, 55 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 42 kDa could potentially be a cleaved form of Protein Z due to the presemce of a 23 amino acid signal peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Protein Z antibody (ab85317)
ICC/IF image of ab85317 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85317 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
