Anti-Prosurfactant Protein C antibody (ab40879)
Key features and details
- Rabbit polyclonal to Prosurfactant Protein C
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Prosurfactant Protein C antibody
See all Prosurfactant Protein C primary antibodies -
Description
Rabbit polyclonal to Prosurfactant Protein C -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human Prosurfactant Protein C aa 1-35.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Prosurfactant Protein C antibody (ab40879)Anti-Prosurfactant Protein C antibody (ab40879) at 1/500 dilution + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Additional bands at: 38 kDa, 49 kDa, 79 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 secondsSecondary antibody - goat anti rabbit HRP (pre-adsorbed-ab97080)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody (ab40879) Image from Raredon et al, BioResearch Open Access, 1, 72-83, Fig. 3e; doi: 10.1089/biores.2016.0006 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0Immunohistochemical analysis of Pig lung tissue, labeling prosurfactant protein C with ab40879. Tissue fixed in 10% buffered formalin for 4 hours, and paraffin embedded. Anitgen retrieval performed in buffer at pH 9 at 75°C for 20 minutes. Blocked with PBS, treated with primary antibody, followed by secondary, and DAPI stained.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody (ab40879)Adult mouse lung was embedded in paraffin and stained with anti-N terminal proSP-C rabbit serum ab40879 (1/2000 dilution).
