Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)
![Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)](https://www.abcam.com/ps/products/254/ab254275/Images/ab254275-369701-AntiProperdin-PFC-3A3E1-western-blot-plasma-serum-human-mouse-rat.jpg "Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [3A3E1] to Properdin/PFC - BSA and Azide free
- Suitable for: WB, ELISA
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free
See all Properdin/PFC primary antibodies -
Description
Mouse monoclonal [3A3E1] to Properdin/PFC - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- WB: Human, mouse and rat plasma lysate. Human and mouse serum lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab254275 is the carrier-free version of ab252531. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3A3E1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)All lanes : Anti-Properdin/PFC antibody [3A3E1] (ab252531) at 0.454 µg/ml
Lane 1 : Human plasma lysate
Lane 2 : Human serum lysate
Lane 3 : Mouse plasma lysate
Lane 4 : Mouse serum lysate
Lane 5 : Rat plasma lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution
Predicted band size: 51 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24797388).
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab252531).
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![ELISA - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)](https://www.abcam.com/ps/products/254/ab254275/Images/ab254275-369702-AntiProperdin-PFC-3A3E1-direct-elisa-dose-response.jpg)
ELISA - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)ELISA - Anti-Properdin/PFC antibody [3A3E1] (ab252531).
Antigen: Human Properdin 1000 ng/ml.
ab252531 used at 0-5000 ng/ml.
Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) secondary used at 1/1000 dilution.
This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab252531).
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![Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)](https://www.abcam.com/ps/products/254/ab254275/Images/ab254275-3-benefits-of-recombinant-antibodies.png)
Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)
