Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [3A3E1] to Properdin/PFC - BSA and Azide free
  • Suitable for: WB, ELISA
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free
    See all Properdin/PFC primary antibodies

  • Description

    Mouse monoclonal [3A3E1] to Properdin/PFC - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Full length native protein (purified). This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human, mouse and rat plasma lysate. Human and mouse serum lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab254275 is the carrier-free version of ab252531. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    3A3E1

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

Images

  • Western blot - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)
    Western blot - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)
    All lanes : Anti-Properdin/PFC antibody [3A3E1] (ab252531) at 0.454 µg/ml

    Lane 1 : Human plasma lysate
    Lane 2 : Human serum lysate
    Lane 3 : Mouse plasma lysate
    Lane 4 : Mouse serum lysate
    Lane 5 : Rat plasma lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

    Predicted band size: 51 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24797388).

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab252531).

  • ELISA - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)
    ELISA - Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)

    ELISA - Anti-Properdin/PFC antibody [3A3E1] (ab252531).

    Antigen: Human Properdin 1000 ng/ml.

    ab252531 used at 0-5000 ng/ml.

    Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) secondary used at 1/1000 dilution.

    This image was produced using the same antibody clone but in a different formulation containing PBS, sodium azide, glycerol and BSA (ab252531).

  • Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)
    Anti-Properdin/PFC antibody [3A3E1] - BSA and Azide free (ab254275)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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