Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] - BSA and Azide free (ab247409)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1516Y] to Progesterone Receptor (phospho S190) - BSA and Azide free
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
Overview
-
Product name
Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] - BSA and Azide free
See all Progesterone Receptor primary antibodies -
Description
Rabbit monoclonal [EP1516Y] to Progesterone Receptor (phospho S190) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects Progesterone Receptor phophorylated at serine 190.
-
Tested applications
Suitable for: ICC, IHC-P, WBmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab247409 is the carrier-free version of ab75857 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247409 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1516Y -
Isotype
IgG -
Research areas
Images
-
This data was developed using ab75857, the same antibody clone in a different buffer formulation.ab75857 at 1/100 dilution staining Progesterone Receptor in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
All lanes : Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857) at 1/10000 dilution
Lane 1 : T47D cell lysates, untreated
Lane 2 : T47D cell lysates, treated with progesterone
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 99 kDa
Observed band size: 118,99 kDa why is the actual band size different from the predicted?This data was developed using ab75857, the same antibody clone in a different buffer formulation.
-