Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRKAR1A (phospho S83 + S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit Monoclonal [EPMAYR1-111] to PRKAR1A (phospho S83 + S83) - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-PRKAR1A (phospho S83 + S83) antibody [EPMAYR1-111] - BSA and Azide free
    See all PRKAR1A primary antibodies

  • Description

    Rabbit Monoclonal [EPMAYR1-111] to PRKAR1A (phospho S83 + S83) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects Protein Kinase A regulatory subunit I alpha/PRKAR1A phosphorylated at serine 83.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human PRKAR1A (phospho S83). The exact sequence is proprietary.
    Database link: P10644

  • General notes

    ab249153 is the carrier-free version of ab154851 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab249153 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Ozgur Ogut at the Mayo Clinic. Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
    Western blot - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
    All lanes : Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] (ab154851) at 1/1000 dilution

    Lane 1 : Rat heart lysate; untreated
    Lane 2 : Rat heart lysate; treated with alkaline phosphatase

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 43 kDa



    This data was developed using ab154851, the same antibody clone in a different buffer formulation.

    Lower panel shows labeling with a non-phospho Protein Kinase A regulatory subunit I alpha/PRKAR1A antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)

    This data was developed using ab154851, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Protein Kinase A regulatory subunit I alpha/PRKA1A with ab154851 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
    This data was developed using ab154851, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Protein Kinase A regulatory subunit I alpha/PRKAR1A with ab154851 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
    This data was developed using ab154851, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154851 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
    This data was developed using ab154851, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab154851 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
    This data was developed using ab154851, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab154851 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)
    Anti-PRKAR1A (phospho S83) antibody [EPMAYR1-111] - BSA and Azide free (ab249153)

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