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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKA

Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)

Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPMAYR1-127] to PRKAR1A (phospho S77) - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] (ab139682)

Overview

  • Product name

    Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free
    See all PRKAR1A primary antibodies
  • Description

    Rabbit monoclonal [EPMAYR1-127] to PRKAR1A (phospho S77) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects Protein Kinase A regulatory subunit I alpha/PRKAR1A phosphorylated at serine 77.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248879 is the carrier-free version of ab139682 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248879 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Ozgur Ogut at the Mayo Clinic.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPMAYR1-127
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKA
    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • cAMP
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Images

  • Western blot - Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)
    Western blot - Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)
    All lanes : Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] (ab139682) at 1/1000 dilution

    Lane 1 : Fetal heart lysate
    Lane 2 : Fetal heart lysate treated with Alkaline Phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 43 kDa



    This data was developed using ab139682, the same antibody clone in a different buffer formulation.

     

  • Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)
    Anti-PRKAR1A (phospho S77) antibody [EPMAYR1-127] - BSA and Azide free (ab248879)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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