Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PR3 antibody [EPR23253-35] (ab270441)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23253-35] to PR3
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PR3 antibody [EPR23253-35]
    See all PR3 primary antibodies

  • Description

    Rabbit monoclonal [EPR23253-35] to PR3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    IHC-P
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human bone marrow, lung, spleen and placenta tissue; Mouse lung and spleen tissue. ICC/IF: Mouse bone marrow cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)

    Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on human bone marrow (PMID: 22247758).The section was incubated with ab270441 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PR3 with ab270441 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in mouse spleen. The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] (ab270441)
    Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] (ab270441)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse bone marrow or EL4 cells labelling PR3 with ab270441 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in a subset of mouse bone marrow cells. Negative control: EL4 (PMID: 9211743). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PR3 with ab270441 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in mouse lung (PMID: 22247758).The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human placenta.The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human spleen.The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] (ab270441)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human lung (PMID: 22247758).The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-PR3 antibody [EPR23253-35] (ab270441)
    Anti-PR3 antibody [EPR23253-35] (ab270441)

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