Anti-PPP6C/Ppv antibody [EPR8764] (ab131335)
![Anti-PPP6C/Ppv antibody [EPR8764] (ab131335)](https://www.abcam.com/ps/products/131/ab131335/Images/ab131335-145729-anti-ppp6c-antibody-epr8764-western-blot.jpg "Anti-PPP6C/Ppv antibody [EPR8764] (ab131335)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8764] to PPP6C/Ppv
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-PPP6C/Ppv antibody [EPR8764]
See all PPP6C/Ppv primary antibodies -
Description
Rabbit monoclonal [EPR8764] to PPP6C/Ppv -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
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Immunogen
corresponding to Human PPP6C/Ppv aa 250-350 (C terminal).
Database link: O00743 -
Positive control
- WB: HeLa, fetal thymus and 293T and Jurkat whole cell lysate (ab7899). ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
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General notes
This product was previously labelled as PPP6C
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.00 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8764 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPP6C/Ppv antibody [EPR8764] (ab131335)All lanes : Anti-PPP6C/Ppv antibody [EPR8764] (ab131335) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Fetal thymus lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 35 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-PPP6C/Ppv antibody [EPR8764] (ab131335)](https://www.abcam.com/ps/products/131/ab131335/Images/ab131335-145728-anti-ppp6c-antibody-epr8764-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PPP6C/Ppv antibody [EPR8764] (ab131335)Immunofluorescent analysis of HeLa cells labelling PPP6C/Ppv with ab131335 at 1/100 dilution.
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![Flow Cytometry - Anti-PPP6C/Ppv antibody [EPR8764] (ab131335)](https://www.abcam.com/ps/products/131/ab131335/Images/ab131335-271127-anti-ppp6c-antibody-epr8764-flow-cytometry.jpg)
Flow Cytometry - Anti-PPP6C/Ppv antibody [EPR8764] (ab131335)Flow Cytometry analysis of HeLa cells labelling PPP6C/Ppv with purified ab131335 at a dilution of 1/280 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
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![OI-RD Scanning - Anti-PPP6C/Ppv antibody [EPR8764] (ab131335)](https://www.abcam.com/ps/products/131/ab131335/Images/ab131335-145727-anti-ppp6c-antibody-epr8764.jpg)
OI-RD Scanning - Anti-PPP6C/Ppv antibody [EPR8764] (ab131335) -
![Anti-PPP6C/Ppv antibody [EPR8764] (ab131335)](https://www.abcam.com/ps/products/131/ab131335/Images/ab131335-5-benefits-of-recombinant-antibodies.png)
Anti-PPP6C/Ppv antibody [EPR8764] (ab131335)
