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Cancer Tumor biomarkers Enzymes Phosphatases

Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)

Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15617] to PPP2R5D - BSA and Azide free
  • Suitable for: ICC, IP, IHC-P, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free
    See all PPP2R5D primary antibodies
  • Description

    Rabbit monoclonal [EPR15617] to PPP2R5D - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431, MCF7, C6, HepG2, Jurkat, HeLa, 293T cell lysate. IHC-P: Human brain tissue. IP: HEK293 cells. Flow Cyt: HeLa cells.
  • General notes

    Ab250952 is the carrier-free version of ab188323. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250952 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR15617
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Phosphatases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Phosphatases

Images

  • Western blot - Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    Western blot - Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    All lanes : Anti-PPP2R5D antibody [EPR15617] (ab188323) at 1/10000 dilution

    Lane 1 : Wild-type A431 cell lysate
    Lane 2 : PPP2R5D knockout A431 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 60-65 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab188323).

    Lanes 1 - 4: Merged signal (red and green). Green - ab188323 observed at 60-65 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab188323 was shown to react with PPP2R5D in wild-type A431 cells in western blot with loss of signal observed in PPP2R5D knockout sample. Wild-type and PPP2R5D knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab188323 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    This data was developed using ab188323, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PPP2R5D using ab188323 at 1/100 dilution. A Ready to use HRP Polymer for Rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    Western blot - Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    All lanes : Anti-PPP2R5D antibody [EPR15617] (ab188323) at 1/100000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 70 kDa
    Observed band size: 70 kDa



    This data was developed using ab188323, the same antibody clone in a different buffer formulation.

    Based on the sequence analysis, ab188323 recognizes 3 isoforms with the predicted MWs of 58KDa, 66KDa and 70KDa, respectively.

  • Immunoprecipitation - Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    Immunoprecipitation - Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    This data was developed using ab188323, the same antibody clone in a different buffer formulation.Immunoprecipitation analysis of 293T cell lysate labeling PPP2R5D using ab188323 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 was used as secondary antibody.
  • Immunocytochemistry - Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    Immunocytochemistry - Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    This data was developed using ab188323, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HepG2 cells labeling PPP2R5D using ab188323 at 1/250 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% Paraformaldehyde. Counterstain: DAPI.
  • Flow Cytometry - Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    Flow Cytometry - Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    This data was developed using ab188323, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa cells labeling PPP2R5D using ab188323 at 1/40 dilution (pink). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG (green).
  • Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)
    Anti-PPP2R5D antibody [EPR15617] - BSA and Azide free (ab250952)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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