Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)
![Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)](https://www.abcam.com/ps/products/249/ab249465/Images/ab249465-445743-anti-ppp2cb-antibody-epr11786-b-bsa-and-azide-free-western-blot.jpg "Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11786(B)] to PPP2CB - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC, WB, IP
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free
See all PPP2CB primary antibodies -
Description
Rabbit monoclonal [EPR11786(B)] to PPP2CB - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC, WB, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human PPP2CB.
Database link: P62714 -
General notes
ab249465 is the carrier-free version of ab168371.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11786(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)
This data was developed using ab168371, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: PPP2CB knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)
ab168371 was shown to specifically react with PPP2CB in wild-type HAP1 cells. No band was observed when PPP2CB knockout samples were examined. Wild-type and PPP2CB knockout samples were subjected to SDS-PAGE. ab168371 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Immunoprecipitation - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)](https://www.abcam.com/ps/products/249/ab249465/Images/ab249465-4-anti-ppp2cb-antibody-epr11786-b-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)This data was developed using ab168371, the same antibody clone in a different buffer formulation.Detection of PPP2CB by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab168371 at 1/10 dilution.
For WB detection ab168371 at 1/10000 dilution was used.
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. -
![Flow Cytometry - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)](https://www.abcam.com/ps/products/249/ab249465/Images/ab249465-3-anti-ppp2cb-antibody-epr11786-b-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465) -
![Immunocytochemistry - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)](https://www.abcam.com/ps/products/249/ab249465/Images/ab249465-2-anti-ppp2cb-antibody-epr11786-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465) -
![Western blot - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)](https://www.abcam.com/ps/products/249/ab249465/Images/ab249465-398321-anti-ppp2cb-antibody-epr11786-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)All lanes : Anti-PPP2CB antibody [EPR11786(B)] (ab168371) at 1/10000 dilution
Lane 1 : 293T lysate
Lane 2 : HeLa lysate
Lane 3 : Human fetal brain lysate
Lane 4 : Human fetal heart lysate
Lane 5 : NIH3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 36 kDaThis data was developed using ab168371, the same antibody clone in a different buffer formulation.
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![Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)](https://www.abcam.com/ps/products/249/ab249465/Images/ab249465-5-benefits-of-recombinant-antibodies.png)
Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (ab249465)