Anti-PPAR alpha antibody - ChIP Grade (ab227074)
Key features and details
- Rabbit polyclonal to PPAR alpha - ChIP Grade
- Suitable for: WB, IP, ChIP
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PPAR alpha antibody - ChIP Grade
See all PPAR alpha primary antibodies -
Description
Rabbit polyclonal to PPAR alpha - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ChIPmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Dog, Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human PPAR alpha (N terminal). The exact sequence is proprietary.
Database link: Q07869 -
Positive control
- ChIP: HepG2 chromatin extract. IP: HepG2 whole cell lysate (ab7900). WB: Rat primary hepatocyte lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Images
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Western blot - Anti-PPAR alpha antibody - ChIP Grade (ab227074)Anti-PPAR alpha antibody - ChIP Grade (ab227074) at 1/1000 dilution + Rat primary hepatocyte lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 52 kDa7.5% SDS-PAGE gel.
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ChIP - Anti-PPAR alpha antibody - ChIP Grade (ab227074)Cross-linked ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab227074. The precipitated DNA was detected by PCR with primer set targeting to C3 promotor or NOD1 gene.
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Immunoprecipitation - Anti-PPAR alpha antibody - ChIP Grade (ab227074)PPAR alpha was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with ab227074 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab227074 at 1/1000 dilution.
Lane 1: Control IgG IP in HepG2 whole cell extract.
Lane 2: ab227074 IP in HepG2 whole cell extract.
