Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 90 460 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23314-256] to Polymeric immunoglobulin receptor/PIGR
Suitable for: IHC-P, WB, IP
Reacts with: Human

Product name

Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256]
See all Polymeric immunoglobulin receptor/PIGR primary antibodies
Description

Rabbit monoclonal [EPR23314-256] to Polymeric immunoglobulin receptor/PIGR
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human colon and colon cancer tissue lysates; His-tagged recombinant human Polymeric immunoglobulin receptor/PIGR protein. IHC-P: Human colon, colon carcinoma and kidney tissue. IP: Human colon tissue lysate.
General notes
This antibody (ab275020, clone EPR23314-256) is a different clone to ab272728 (clone EPR23314-78).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23314-256
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human colon carcinoma (PMID: 19338768). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

All lanes : Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) at 1/1000 dilution

Lane 1 : Human colon tissue lysate
Lane 2 : Human colon cancer tissue lysate
Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

Predicted band size: 83 kDa
Observed band size: 85-100 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 10 seconds.

Low expression cell line: A549 (PMID:21965228).
Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

Polymeric immunoglobulin receptor/PIGR was immunoprecipitated from 0.35 mg Human colon tissue lysate with ab275020 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275020 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Human colon tissue lysate 10 ug

Lane 2: ab275020 IP in Human colon tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275020 in human colon tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human colonic mucosa (PMID: 19338768). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) at 1/1000 dilution + His-tagged recombinant human Polymeric immunoglobulin receptor/PIGR protein 10 ng

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 100000 cells

Predicted band size: 83 kDa

Blocking and dilution buffer:5% NFDM/TBST.

Exposure time: 6 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human kidney (PMID: 31404540). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

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