Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23314-256] to Polymeric immunoglobulin receptor/PIGR
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256]
    See all Polymeric immunoglobulin receptor/PIGR primary antibodies

  • Description

    Rabbit monoclonal [EPR23314-256] to Polymeric immunoglobulin receptor/PIGR

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human colon and colon cancer tissue lysates; His-tagged recombinant human Polymeric immunoglobulin receptor/PIGR protein. IHC-P: Human colon, colon carcinoma and kidney tissue. IP: Human colon tissue lysate.

  • General notes

    This antibody (ab275020, clone EPR23314-256) is a different clone to ab272728 (clone EPR23314-78).

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23314-256

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human colon carcinoma (PMID: 19338768). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
    Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
    All lanes : Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) at 1/1000 dilution

    Lane 1 : Human colon tissue lysate
    Lane 2 : Human colon cancer tissue lysate
    Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 83 kDa
    Observed band size: 85-100 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Low expression cell line: A549 (PMID:21965228).

  • Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
    Immunoprecipitation - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

    Polymeric immunoglobulin receptor/PIGR was immunoprecipitated from 0.35 mg Human colon tissue lysate with ab275020 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275020 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Human colon tissue lysate 10 ug

    Lane 2: ab275020 IP in Human colon tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275020 in human colon tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human colonic mucosa (PMID: 19338768). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
    Western blot - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
    Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020) at 1/1000 dilution + His-tagged recombinant human Polymeric immunoglobulin receptor/PIGR protein 10 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 100000 cells

    Predicted band size: 83 kDa



    Blocking and dilution buffer:5% NFDM/TBST.

    Exposure time: 6 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Polymeric immunoglobulin receptor/PIGR with ab275020 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human kidney (PMID: 31404540). The section was incubated with ab275020 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)
    Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-256] (ab275020)

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