Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PML Protein (phospho S518) antibody [EPR6151] - BSA and Azide free (ab184769)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6151] to PML Protein (phospho S518) - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PML Protein (phospho S518) antibody [EPR6151] - BSA and Azide free
    See all PML Protein primary antibodies

  • Description

    Rabbit monoclonal [EPR6151] to PML Protein (phospho S518) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects PML Protein phosphorylated at Serine 518.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa lysate, Human astrocytoma or breast carcinoma tissue.

  • General notes

    ab184769 is the carrier-free version of ab128932 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab184769 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PML Protein (phospho S518) antibody [EPR6151] - BSA and Azide free (ab184769)
    Western blot - Anti-PML Protein (phospho S518) antibody [EPR6151] - BSA and Azide free (ab184769)
    All lanes : Anti-PML Protein (phospho S518) antibody [EPR6151] (ab128932) at 1/1000 dilution

    Lane 1 : HeLa lysate (untreated)
    Lane 2 : HeLa lysate treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 110 kDa
    Observed band size: 110 kDa



    This data was developed using ab128932, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PML Protein (phospho S518) antibody [EPR6151] - BSA and Azide free (ab184769)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PML Protein (phospho S518) antibody [EPR6151] - BSA and Azide free (ab184769)
    This data was developed using ab128932, the same antibody clone in a different buffer formulation.ab128932, at a 1/100 dilution, staining Human PML Protein in astrocytoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PML Protein (phospho S518) antibody [EPR6151] - BSA and Azide free (ab184769)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PML Protein (phospho S518) antibody [EPR6151] - BSA and Azide free (ab184769)
    This data was developed using ab128932, the same antibody clone in a different buffer formulation.ab128932, at a 1/50 dilution, staining Human PML Protein in breast carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Anti-PML Protein (phospho S518) antibody [EPR6151] - BSA and Azide free (ab184769)
    Anti-PML Protein (phospho S518) antibody [EPR6151] - BSA and Azide free (ab184769)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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