Anti-PMIS2 antibody (ab194880)
Key features and details
- Rabbit polyclonal to PMIS2
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-PMIS2 antibody -
Description
Rabbit polyclonal to PMIS2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse PMIS2 aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: Q497Q9 -
Positive control
- Wild-type mouse testis and sperm lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-PMIS2 antibody (ab194880)Anti-PMIS2 antibody (ab194880) at 1/1000 dilution + Mouse sperm lysate
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 11 kDaSDS-PAGE (10~20% gradient gel).
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Western blot - Anti-PMIS2 antibody (ab194880)Western blot analysis of PMIS2 protein expression in the lysates of testis and sperm from wild-type (+/+) and PMIS2 knockout (-/-) mice using ab194880 at 1/1000 dilution.
The difference in predicted molecular mass (11 kDa) and the band position (18 kDa) of PMIS protein might be due to glycosylation.
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Immunohistochemistry (Paraffin-embedded sections) - Anti-PMIS2 antibody (ab194880)Immunohistochemical analysis of paraffin embedded mouse testis tissue labeling PMIS2 with ab194880 at 1/1000.
Secondary antibody - Goat anti-rabbit IgG Alexa Fluor 488 conjugated at 1/1000 for 60 minutes.
DAPI staining for 10 minutes at 1 µg/mL in TBS.
Blocking with 10% FBS/PBST for 30 mins.
Washing steps done with PBST (0.25% triton X- 100/PBS) and PBS.