Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)
![Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)](https://www.abcam.com/ps/products/193/ab193355/Images/ab193355-366534-anti-plexin-b2-antibody-epr9965-western-blot-knockout-hek-293-plxnb2-hela-human-brain-human-lysates.jpg "Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9965] to Plexin B2/MM1
- Suitable for: Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Plexin B2/MM1 antibody [EPR9965]
See all Plexin B2/MM1 primary antibodies -
Description
Rabbit monoclonal [EPR9965] to Plexin B2/MM1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB RatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Human fetal brain, U87-MG, rat brain, RAW264.7, NIH/3T3 and 293T lysate. U87-MG cells.
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General notes
Previously labelled as Plexin B2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9965 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)All lanes : Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/5000 dilution
Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : PLXNB2 knockout HEK-293 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Human brain whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 204 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab193355 observed at 80 kDa. Red - loading control, ab7291, observed at 50 kDa.
ab193355 was shown to specifically react with in wild-type HEK-293 cells as signal was lost in PLXNB2 knockout cells. Wild-type and PLXNB2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab193355 and ab7291 (Mouse anti-Tubulin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)](https://www.abcam.com/ps/products/193/ab193355/Images/ab193355-230978-anti-plexin-b2-mm1-antibody-epr9965-western-blot.jpg)
Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/10000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 204 kDaPlexin B2/MM1 could be cleaved into two subunits. The band observed at 80 kDa should be a β-chain.
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![Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)](https://www.abcam.com/ps/products/193/ab193355/Images/ab193355-230977-anti-plexin-b2-mm1-antibody-epr9965-western-blot.jpg)
Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)All lanes : Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : U87-MG cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 204 kDa
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![Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)](https://www.abcam.com/ps/products/193/ab193355/Images/ab193355-230976-anti-plexin-b2-mm1-antibody-epr9965-western-blot.jpg)
Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)All lanes : Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Raw264.7 lysate
Lane 3 : NIH/3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 204 kDa
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![Flow Cytometry - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)](https://www.abcam.com/ps/products/193/ab193355/Images/ab193355-230975-anti-plexin-b2-mm1-antibody-epr9965-flow-cytometry.jpg)
Flow Cytometry - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)Flow Cytometry analysis of U87-MG cells labeling Plexin B2/MM1 using ab193355 at a 1/90 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled control/ colour cells without incubation with primary antibody/ secondary antibody (blue). Cells were fixed in 2% paraformaldehyde.
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![Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)](https://www.abcam.com/ps/products/193/ab193355/Images/ab193355-6-benefits-of-recombinant-antibodies.png)
Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)
