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Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9965] to Plexin B2/MM1
  • Suitable for: Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Plexin B2/MM1 antibody [EPR9965]
    See all Plexin B2/MM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9965] to Plexin B2/MM1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, Human fetal brain, U87-MG, rat brain, RAW264.7, NIH/3T3 and 293T lysate. U87-MG cells.

  • General notes

    Previously labelled as Plexin B2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)
    Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)
    All lanes : Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/5000 dilution

    Lane 1 : Wild-type HEK-293 whole cell lysate
    Lane 2 : PLXNB2 knockout HEK-293 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : Human brain whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 204 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab193355 observed at 80 kDa. Red - loading control, ab7291, observed at 50 kDa.

    ab193355 was shown to specifically react with in wild-type HEK-293 cells as signal was lost in PLXNB2 knockout cells. Wild-type and PLXNB2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab193355 and ab7291 (Mouse anti-Tubulin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)
    Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)
    Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/10000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 204 kDa



    Plexin B2/MM1 could be cleaved into two subunits. The band observed at 80 kDa should be a β-chain.

  • Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)
    Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)
    All lanes : Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : U87-MG cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 204 kDa

  • Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)
    Western blot - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)
    All lanes : Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Raw264.7 lysate
    Lane 3 : NIH/3T3 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 204 kDa

  • Flow Cytometry - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)
    Flow Cytometry - Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)

    Flow Cytometry analysis of U87-MG cells labeling Plexin B2/MM1 using ab193355 at a 1/90 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (black). Unlabeled control/ colour cells without incubation with primary antibody/ secondary antibody (blue). Cells were fixed in 2% paraformaldehyde.

  • Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)
    Anti-Plexin B2/MM1 antibody [EPR9965] (ab193355)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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