Anti-Plakophilin 1 antibody [EPR14890] (ab183512)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14890] to Plakophilin 1
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-Plakophilin 1 antibody [EPR14890]
See all Plakophilin 1 primary antibodies -
Description
Rabbit monoclonal [EPR14890] to Plakophilin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB MouseHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Mouse skin, Human skin and Human tonsil tissue lysates and A431 and HACAT cell lysates; HACAT cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14890 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-Plakophilin 1 antibody [EPR14890] (ab183512) at 1/20000 dilution
Lane 1 : Mouse skin cell lysate
Lane 2 : A431 cell lysate
Lane 3 : HACAT cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
-
All lanes : Anti-Plakophilin 1 antibody [EPR14890] (ab183512) at 1/5000 dilution
Lane 1 : Human skin tissue lysate
Lane 2 : Human tonsil tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
-
Immunofluorescent staining of 4% paraformaldehyde fixed HACAT cells labeling Plakophilin 1 using ab183512 at a 1/100 dilution followed by Goat anti Rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution. Counterstained with Dapi (bue).
-
Flow cytometric analysis of HACAT cells (2% paraformaldehyde fixed) labeling Plakophilin 1 with ab183512 at 1/90 dilution (red) compared to a Rabbit monoclonal isotype control (green).
-