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Developmental Biology Organogenesis Gut development Gut specification

Anti-PKHD1 antibody [8G12A1] (ab204951)

Anti-PKHD1 antibody [8G12A1] (ab204951)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rat monoclonal [8G12A1] to PKHD1
  • Suitable for: ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-PKHD1 antibody [8G12A1]
    See all PKHD1 primary antibodies
  • Description

    Rat monoclonal [8G12A1] to PKHD1
  • Host species

    Rat
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Mouse PKHD1 aa 3850-4100.
    Database link: Q80ZA4

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Mouse recombinant protein, A431 cells, HeLa cells, esophageal cancer tissue.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR291788-5 are from Tissue Culture Supernatant

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    8G12A1
  • Isotype

    IgG1
  • Research areas

    • Developmental Biology
    • Organogenesis
    • Gut development
    • Gut specification
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Liver development
    • Developmental Biology
    • Organogenesis
    • Excretory system development
    • Kidney development

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PKHD1 antibody [8G12A1] (ab204951)
    Immunocytochemistry/ Immunofluorescence - Anti-PKHD1 antibody [8G12A1] (ab204951)

    Immunofluorescent analysis of A431 cells labeling PKHD1 with ab204951 at 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

  • Flow Cytometry - Anti-PKHD1 antibody [8G12A1] (ab204951)
    Flow Cytometry - Anti-PKHD1 antibody [8G12A1] (ab204951)

    Flow cytometric analysis of HeLa cells labeling PKHD1 with ab204951 at 1/200 (green) or negative control (red)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKHD1 antibody [8G12A1] (ab204951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKHD1 antibody [8G12A1] (ab204951)

    Immunohistochemical analysis of paraffin-embedded esophageal cancer tissue labeling PKHD1 with ab204951 at 1/200 dilution followed by DAB staining

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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