Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)
![Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)](https://www.abcam.com/ps/products/247/ab247449/Images/ab247449-445560-anti-pkc-delta-phospho-y311-antibody-epr2609y-bsa-and-azide-free-western-blot.jpg "Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2609Y] to PKC delta (phospho Y311) - BSA and Azide free
- Suitable for: Dot blot, IHC-P, IP, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free
See all PKC delta primary antibodies -
Description
Rabbit monoclonal [EPR2609Y] to PKC delta (phospho Y311) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody only detects PKC delta phosphorylated on Tyrosine 311.
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Tested applications
Suitable for: Dot blot, IHC-P, IP, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247449 is the carrier-free version of ab76181 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247449 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2609Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)All lanes : Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181) at 1/500 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate at 200nM for 20 minutes. Whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate at 200nM for 20 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab76181, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)](https://www.abcam.com/ps/products/247/ab247449/Images/ab247449-4-anti-pkc-delta-phospho-y311-antibody-epr2609y-bsa-and-azide-free-dot-blot.jpg)
Dot Blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)This data was developed using ab76181, the same antibody clone in a different buffer formulation.Dot blot analysis of PKC delta (pY311) phospho peptide (Lane 1) and PKC delta non-phospho peptide (Lane 2) using ab76181 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution. Blocking and Diluting buffer and concentration: 5% NFDM /TBST. Exposure time: 3 minutes. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)](https://www.abcam.com/ps/products/247/ab247449/Images/ab247449-3-anti-pkc-delta-phospho-y311-antibody-epr2609y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449) -
![Immunoprecipitation - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)](https://www.abcam.com/ps/products/247/ab247449/Images/ab247449-406340-anti-pkc-delta-phospho-y311-antibody-epr2609y-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)This data was developed using ab76181, the same antibody clone in a different buffer formulation.
ab76181 at 1/20 immunoprecipitating PKC delta (phospho Y311) in HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
Lane 1 (input): HeLa treated with 10mM H2O2 for 1 hour whole cell lysate (10µg)
Lane 2 (+): ab76181 + HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76181 in HeLa treated with 10mM H2O2 for 1 hour whole cell lysate.
For western blotting, ab76181 was used as a dilution of 1/200 followed by ab131366 VeriBlot for IP (HRP) at a dilution of 1/1000.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)](https://www.abcam.com/ps/products/247/ab247449/Images/ab247449-402336-anti-pkc-delta-phospho-y311-antibody-epr2609y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)All lanes : Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181) at 1/5000 dilution
Lane 1 : U937 cell lysate
Lane 2 : U937 cell lysate treated with TPA
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit-HRP at 1/2000 dilution
Predicted band size: 77 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?This data was developed using ab76181, the same antibody clone in a different buffer formulation.
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![Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)](https://www.abcam.com/ps/products/247/ab247449/Images/ab247449-5-benefits-of-recombinant-antibodies.png)
Anti-PKC delta (phospho Y311) antibody [EPR2609Y] - BSA and Azide free (ab247449)